Scientists decipher the nanoscale architecture of a beetle's shell

Ruiguo Yang, assistant professor of mechanical and materials engineering at the University of Nebraska-Lincoln, and his colleagues found a way to analyze the fibrous nanostructure of a beetle's lightweight but durable shell.

Beetles wear a body armor that should weigh them down -- think medieval knights and turtles. In fact, those hard shells protecting delicate wings are surprisingly light, allowing even flight.

Better understanding the structure and properties of beetle exoskeletons could help scientists engineer lighter, stronger materials. Such materials could, for example, reduce gas-guzzling drag in vehicles and airplanes and reduce the weight of armor, lightening the load for the 21st-century knight.

But revealing exoskeleton architecture at the nanoscale has proven difficult. Nebraska's Ruiguo Yang, assistant professor of mechanical and materials engineering, and his colleagues found a way to analyze the fibrous nanostructure. Their findings were featured recently on the cover of Advanced Functional Materials.

The lightweight exoskeleton is composed of chitin fibers just around 20 nanometers in diameter (a human hair measures approximately 75,000 nanometers in diameter) and packed and piled into layers that twist in a spiral, like a spiral staircase. The small diameter and helical twisting, known as Bouligand, make the structure difficult to analyze.

Yang and his team developed a method of slicing down the spiral to reveal a surface of cross-sections of fibers at different orientations. From that viewpoint, the researchers were able to analyze the fibers' mechanical properties with the aid of an atomic force microscope. This type of microscope applies a tiny force to a test sample, deforms the sample and monitors the sample's response. Combining the experimental procedure and theoretical analysis, the researchers were able to reveal the nanoscale architecture of the exoskeleton and the material properties of the nanofibers.

They made their discoveries in the common figeater beetle, Cotinis mutabilis, a metallic green native of the western United States. But the technique can be used on other beetles and hard-shelled creatures and might also extend to artificial materials with fibrous structures, Yang said.

Comparing beetles with differing demands on their exoskeletons, such as defending against predators or environmental damage, could lead to evolutionary insights as well as a better understanding of the relationship between structural features and their properties.

References

Ruiguo Yang, Alireza Zaheri, Wei Gao, Cheryl Hayashi, Horacio D. Espinosa, Exoskeletons: AFM Identification of Beetle Exocuticle: Bouligand Structure and Nanofiber Anisotropic Elastic Properties (Adv. Funct. Mater. 6/2017), Advanced Functional Materials, 8 February 2017